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xhtmlTechnology Gallery
[2007][2006] [2005] [2003]
Gallery 2007
In September :
New imaging set-up for the Cytocon400 system (Evotec Technologies/Perkin Elmer). Photos are showing the brand new spinning disk confocal Andor Revolution XD (Andor Technology, UK) attached to the side port of the inverted microscope. The whole system is inside a black box (Science Ware, USA). Two cameras are attached to the system, a DV885 for the spinning disk acquisition and a DV887 back illuminated for low light acquisition. The system configuration shown in the photo allow single cell sorting experiments and mirco-rotation imaging.
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In June :
Running experiment using the compact generator CytoSmart from Evotec Technologies (Germany) on a spinning disk confocal Andor Revolution (Andor Technology, UK). Live cells expressing green fluorescent protein are recorded under microrotation.
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Gallery 2006
In September :
These photos show the brand new black-box (from Science Ware, USA) that was added to the widefield set-up. The black-box ensures that no external light can disturb the image acquisition during cell rotation. The black-box is customised for our system allowing to remove front and back panels for better access, moreover design gives the possibility to plug the two mercury light sources (Cairn Research, UK) to the microscpe. Cell rotation can be done using either the Cytocon400 generator or the new compact generator CytoSmart from Evotec Technologies (Germany). Some experiments have done using a spinning disk confocal system from Andor Technology (UK). This system is built with a Yokogawa CSU22 scan head and a EMCCD camera DV885 from Andor Technology.
The black-box on the cell rotator
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The CytoSmart
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The Spinning Disk
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Gallery 2005
In September : The pFRET
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In August : On confocal
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First experiment using the CytoSmart device on a confocal LSM510 Meta from Zeiss. The CytoSmart device is a small box that allow the user to trap and rotate a single cell. The Cytosmart is USB controlled by a laptop computer. The CytoSmart box is shown on the left top and right bottom pictures (green stickers).
In May : After the fisrt automation meeting
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These pictures were taken just after the first automation meeting. We can see that we got new hardware. The 2 lower pictures show the first set-up with new harware. The large square box on the right side of the microscope is the Cytocon 400 (generator). This device is completely controlled through the computer. The transparent box also on the right is the 96 well plate cell sorting. It is also controlled via the software and allows fast sorting of single cells. Photos in the top panel show a working configuration for a cell sorting experiment. The Cytocon 400 has been moved to the left side of the microscope for easier accessibility. The camera has been moved to the bottom port of the microscope to collect more photons.
In March
These photos show the set-up that was made just after the official launch of the Automation project. We used an inverted manual microscope from Zeiss. The left picture shows two different pieces of hardware from Evotec Technologies: the Cytocon 300 that allow the user to do cell sorting and cell rotation (boxes at the right top corner of the picture) and the Cytoman that allows user to do only cell trapping (small box at the right of the microscope). The microfuildic control boxes are shown in the left part of the picture. We used a Andor EM CCD back illuminated camera 512x512 (DV887BCS) that is connected on the left port of the microscope. The camera is controlled using a second computer. The right picture shows a blow-up of the microscope stage with a set-up for cell trapping and rotation. In the middle of the picture, you can see the DFC-3 chip from Evotec Technologies which is connected to the Cytoman. Tubing are going in and out the chips to flow cells.
Gallery 2003
In July
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Spencer Shorte got the idea to use the dielectrophoresis field technology to rotate and image single living cells. Following his idea, Spencer Shorte developed the first prototype based on the trapping system with Thomas Schnelle (Evotec Technologies) and Torsten Müller (Evotec Technologies). They modified the cell sorting system from Evotec Technologies that was present on the Light Microscopy Center of the Institut Pasteur to control the trapping and the rotation of a single living cell. The first movie that has been done with the system can be seen in the Movie Gallery. The technology of rotating and imaging a single cell is patented by Institut Pasteur and Evotec Technologies. The system was build on a inverted Zeiss microscope equiped with several cameras: Orca ER from Hamamatsu (shown in these pictures), amplified camera from Hamamatsu and CoolSnapHQ from Ropper Scientific. The bottom right picture shows the DFC-3 chip where cells are flowed. The Top picture show the software interface to control the activation of electrodes inside the chip and a general view of the set-up.
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