Plate-Forme Imagerie Dynamique (PFID)

Plateforme: staff: Dr. Spencer L. Shorte

Areas of interest/expertise:

Cell Biology, Cell Physiology, Endocrinology, Neurophysiology, Cytoskeleton, Calcium signalling, Intracellular Secondary Messenger Signalling, Biochemistry, Digital-imaging techniques.

Degrees

  • 1989-92 University of Bristol, United-Kingdom PhD, Biochemistry
  • 1988-89 University of Bristol, United-Kingdom MSc, Biochemistry
  • 1984-87 University of Kent at Canterbury, United-Kingdom BSc, Biochemistry

Precedent post

Assistant Professor, Medical University of South Carolina

Research publications

Shorte, S.L., Collingridge, G.L., Randall, A.D., Chappell, J.B. & Schofield, J.G. (1991). Ammonium ions mobilise calcium from an internal pool which is insensitive to TRH and ionomycin in bovine anterior pituitary cells. Cell Calcium, 12, 301-312.

Shorte, S.L. & Schofield, J.G. (1991). TRH-induced cytosolic calcium transients: characterisation of store refilling in bovine anterior pituitary cells. Molecular & Cellular Endocrinology, 79, 167-176.

Stafford, S.J.V., Shorte, S.L. & Schofield, J.G. (1992). Use of a fluorescent dye to measure secretion from intact bovine anterior pituitary cells, Bioscience Report, 13, 9-17.

Shorte, S.L., Stafford, S.J.V. Bamford, M., Collett, V.J. & Schofield, J.G. (1993). Thyroliberin-induced changes in the fluorescence of a membrane probe in individual bovine anterior pituitary cells. Journal of Physiology (London), 470, 191-210.

Shorte, S.L., Stafford, S.J.V., Collett, V.J. & Schofield, J.G. (1995). Simultaneous measurement of [Ca2+]i and secretion-coupled membrane turnover by single cell fluorescence microscopy. Cell Calcium, 18, 440-454.

Shorte, S.L. & Schofield, J.G. (1996). The effect of extracellular polyvalent cations on bovine anterior pituitary cells- evidence for a calcium sensing receptor coupled to release of intracellular calcium stores. Cell Calcium, 19, 43-57.

Shorte, S.L. (1997). n-Methyl-d-aspartate evokes a rapid net depolymerisation of filamentous actin in cultured rat cerebellar granule cells. Journal of Neurophysiology (USA). 78, 1135-1143.

Shorte, S.L. & Bolsover, S. (1999). Imaging Reality- understanding maps of physiological cell signals measured by fluorescence microscopy, and digital imaging. In Fluorescent and Luminescent Probes, 2nd edition. Inviting Editor. W.Mason. Pub. Academic Press Ltd. (London).

David-Watine, B., Shorte, .S.L., Fucile. S., De Saint Jan, D. & Bregestovski, P. (1999). Functional integrity of a Green Fluorescent Protein conjugated glycine receptor alpha subunit cloned from zebrafish. Neuropharmacology 38, 785-792.

Shorte, S.L., Faught, W.J. & Frawley, L.S. (2000). Spontaneous calcium oscillatory patterns in mammotropes display non-random dynamics. Cell Calcium, 28, 171-179.

Vazquez-Martinez, R., Shorte, S.L., Faught, W.J., Leaumont, D.C., Frawley, L.S., & Boockfor, F.R. (2001). Synchronized exocytotic bursts from gonadotropin-releasing hormone (GnRH)- expressing cells : Dual control by intrinsic cellular pulsatility and gap junctional communication. Endocrinology, 142, 2095-2101.

Vazquez-Martinez, R., Shorte, S.L., Faught, W.J., Leaumont, D.C., Frawley, L.S., & Boockfor, F.R. (2001). Pulsatile exocytosis is functionally associated with gonadotropin-releasing hormone (GnRH) gene expression in immortalized GnRH expressing cells. Endocrinology, 142, 5364-5370.

Shorte, S. L., Leclerc, G. M., Vazquez-Martinez, R., Leaumont, D. C., Faught, W. J. Frawley, L. S. & Boockfor, F. R. PRL gene expression in individual living mammotropes displays distinct functional pulses that oscillate in a non-circadian temporal pattern. Endocrinology 143, 1126-33 (2002)*

Leclerc, G. M., Leclerc, G. J., Shorte, S. L., Stephen Frawley, L. & Boockfor, F. R. Cloning and mRNA expression of the Ca(2+)-binding DREAM protein in the pituitary. Gen Comp. Endocrinol 129, 45-55 (2002)

Boeda, B., El-Amraoui, A., Bahloul, A., Goodyear, R., Daviet, L., Blanchard, S., Perfettini, I., Fath, K. R., Shorte, S., Reiners, J., Houdusse, A., Legrain, P., Wolfrum, U., Richardson, G., Petit, C. Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle. EMBO J 21, 6689-99 (2002)

Patents

Shorte, S.L., Müller, T., Schnell, T. DI 200243, Invention: "Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy" Patent Number; EP 02 292 658.8; file date: 25 October 2002

Addendum

  • 2001 Awarded honorarium prize for "exceptional research" presented at the 83rd meeting of the Endocrine Society (Denver, 2001, see * above).
  • 2002 May: Co-organiser to ELMI (European Light Microscopy Initiative) congress held at IP-Paris, 150 attendees (duties included fundraising).
  • 2002 December: Organiser to AIM (Advanced Light Microscopy) Workshop held at IP-Paris, 60 attendees.
  • 2003 March: Organiser to 1st IP-EAMNET workshop "Dynamic Imaging Microscopy & Analysis for Biologists"


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